Yes, if all three accounts are in the same domain, all three users can access the guide designed by the author user. You can ensure that all three licenses are working within the same common guide's environment by assigning the appropriate roles and permissions to each user.
Regarding tracking different users accessing the guide on the same HoloLens 2 through Power BI, it is possible. You can configure user tracking and analytics within Power BI to monitor and report on the activities of each user accessing the guide.
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